GrainGenes Sequence Report: CA498868
Sequence
CA498868
Contig
Ta.7476.1.S1_at NSFT03P2_Contig8249
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC311608
NCBI UniGene
Ta.7476
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001042486.1 Os01g0229400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat meiotic anther cDNA library
Tissue
Anther
Developmental Stage
Meiotic stages pre-meiosis-metaphase I
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE3248_F10_L20ZT Wheat meiotic anther cDNA library Triticum aestivum cDNA clone WHE3248_F10_L20, mRNA sequence.
Strain
lab_host E. coli DH10B
Clone
WHE3248_F10_L20
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7 primer. Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Plants were grown in a glasshouse. Anther meiotic stage was determined by removing anthers from individual primary florets. One anther was sacrificed for microscopic staging , and if determined to be between (and including) meiotic stages pre-meiosis and metaphase I, the remaining two anthers were collected and pooled for library construction. The tissue, total RNA, and poly(A) RNA were prepared, cDNA synthesised, and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.5Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Plants were grown in a glasshouse. Anther meiotic stage was determined by removing anthers from individual primary florets. One anther was sacrificed for microscopic staging, and if determined to be between (and including) meiotic stages pre-meiosis and metaphase I, the remaining two anthers were collected and pooled for library construction. The tissue, total RNA, and poly(A) RNA were prepared, cDNA synthesised, and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.5Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
catcaagtgcgtggccgtgggcgacggggccgtgggcaaaacctgcatgc
tcatctgctacacctgcaacaagttccccaccgactacatccccaccgtg
gtcgacaacttcagcgccaatgtctccgtggacgggagcatcgtcaacct
cggcctctgggacaccgcagggcaggaggattacagcagggtgagggctc
tgagctacaggggagccgatggcttcatcctctccttctccctcaccagc
agagcaagctatgagaatgtgcacaagaagtggatgccggagcttcgccg
ggacgcccccggcattcctggactacttggtggaaccaagttggatctcc
gagaagatagagcttatcttgctgatcatgcagctgattccataataaca
actgagcagg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CA498868
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||