GrainGenes Sequence Report: NSFT03P2_Contig13647
Sequence
CA497105
Contig
Ta.7657.1.S1_s_at Ta.7657.2.S1_a_at Ta.7657.3.S1_a_at NSFT03P2_Contig13647
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC308965
NCBI UniGene
Ta.7657
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001060917.1 Os08g0129700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat meiotic anther cDNA library
Tissue
Anther
Developmental Stage
Meiotic stages pre-meiosis-metaphase I
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE3223_B07_D13ZT Wheat meiotic anther cDNA library Triticum aestivum cDNA clone WHE3223_B07_D13, mRNA sequence.
Strain
lab_host E. coli DH10B
Clone
WHE3223_B07_D13
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7 primer. Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Plants were grown in a glasshouse. Anther meiotic stage was determined by removing anthers from individual primary florets. One anther was sacrificed for microscopic staging , and if determined to be between (and including) meiotic stages pre-meiosis and metaphase I, the remaining two anthers were collected and pooled for library construction. The tissue, total RNA, and poly(A) RNA were prepared, cDNA synthesised, and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.5Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Plants were grown in a glasshouse. Anther meiotic stage was determined by removing anthers from individual primary florets. One anther was sacrificed for microscopic staging, and if determined to be between (and including) meiotic stages pre-meiosis and metaphase I, the remaining two anthers were collected and pooled for library construction. The tissue, total RNA, and poly(A) RNA were prepared, cDNA synthesised, and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.5Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ccacgcgtccgcaatagtgtgttttctcgtcatgaacctggggttaccca
tgtcttagtgacaggaggagctggctatattggttcacatgcctcattac
agctattaaaagacaactatcgagttaccattgtggacaatctttctaga
ggaaataaaggagcagtaaaggttctccaagaattatttccggagcctgg
gagacttcaattcatctatgcagatcttggggatcaaaaatctgtcaaca
agatattttctgaaaatgcatttgatgctgtgatgcactttgcggctgtt
gcctatgtgggtgagagtacaatggaaccccttaggtattatcacaatat
tacatcaaacaccttactgattttggaggctatggcttctcatggagtca
agacacttatttactctagtacctgtgcaacttatgg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig13647
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||