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GrainGenes Sequence Report: CA030432

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Sequence
CA030432
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC153896
NCBI UniGene
Hv.25466
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001048656.1 Os03g0101700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HX07A20r HX Hordeum vulgare subsp. vulgare cDNA clone HX07A20 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HX07A20
Probe
GBS802
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 585 Std Error: 0.00; Plate: 7 row: A column: 20; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgagggtacctgccgacctcaagccgcaccagctcgacggcgtcc
actggctcatccgccgctaccacctcggcgtcaacgtcctgctcggcgac
gagatggggctggggaagaccctgcaggcaatttctctgctgagctacct
gaagatcaaatccatcgcccccgggccgttcctggtgttatgccctctga
gcgtgacggatggctggttgtcggagttcggtaaattctgccctacattg
aaggtcatccagtatgtcggtgataaggcgcatcgtcgtcatatacggag
aacgatgcatgacgatgtccaaaaatcttcacattcaaatgaattgccat
ttgatgtgatgctgacgagctatgacatagccttaatggatcaagatttt
ctttcacaaattccttggctctatgtggtcattgatgaggcacaacgtct
taaaaatccttcaagtgtactgtataatgttcttgaagaacgctttatga
tgccaaggcgtctactactaacaggcactcctgtccagaataacctttct
gaactatgggcgttaatgcacttctgcatgccttc

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