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GrainGenes Sequence Report: CA029945

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Sequence
CA029945
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC184932
NCBI UniGene
Hv.7161
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HX05K11r HX Hordeum vulgare subsp. vulgare cDNA clone HX05K11 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HX05K11
Probe
GBM1516
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 649 Std Error: 0.00; Plate: 5 row: K column: 11; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgaggaccgcatccaactagagcaggcctaagcacaccaaccaac
gccccactcgatcccctcatcctctgtacccgacaaaccaggggacaacc
gtcctcgttctgcctcctctcgatctgcagtgtgtcctcctggcacttgc
aggagcatcccgagcgccgtgcagaagttcatcccgagctcctccaacct
cgcgacctcgtcgcgatgaccggagatcaagccgccgtcccttgtccccg
tgctatctccaccactctttcgttttttttctctcccagtttctcaactc
tctctctctctctgtttctctggcaggatgcatcaccggccaccggagct
tctccatggtcccttgggcctcgccccacctccagggactgcctccgcgc
cctctcctttccctatcgtgcctcccgtgtttctccttctcctccctccc
gctcagctctctctctctctctcttgaccaacaggaacatcaaccccacc
atggatggggtgctcgcagcttgtgcaccagcggtccaccgcgaattgat
cccctgacgctggaaatggagcccgtgtcatcggatccaccgcttccgga
ccgcgccaccggacatcatcgccgctgccttcccgttcctcgctgagtt

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