GrainGenes Sequence Report: CA029674

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Sequence

CA029674

External Databases

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf152d04, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

pericarp

Developmental Stage

0-7 DAP (days after pollination)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HZ65O04r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ65O04 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HZ65O04

Probe

BaAL30P12

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 400 Std Error: 0.00; Plate: 65 row: O column: 4; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); pericarp 0-7 DAP(days after pollination). Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp

DNA

gatgataacgcaaaacattcgctcgacgattaaaattcagtttgacaagt
gataaccactgaccacaataggttttttttttgttctcacaaacataaac
actgaccgaagatgttcaccgacaccacctcataagacttgaggataatt
acaacccattacgacgacataaagaagagcgctgaaatgcagacatgaca
aatactaagagttggtcattcagcacagcggcagcggcaccacctagatg
ctcctctactcaaaggcccactggttctccctgcggatctcctcctcctc
ctcgggagtaaagtcattcttgatgttgaatgtcttgcggatctcctctg
gggttttgcccttgatcctgaccagcacaccccgatctttcgctctctcg


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