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GrainGenes Sequence Report: CA028897

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Sequence
CA028897
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC158020
NCBI UniGene
Hv.3736
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf108g19, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HZ
Tissue
pericarp
Developmental Stage
0-7 DAP (days after pollination)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HZ63J03r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ63J03 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HZ63J03
Probe
GBS759
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 399 Std Error: 0.00; Plate: 63 row: J column: 3; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); pericarp 0-7 DAP(days after pollination). Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp
DNA
ctcggctcagctcccctctctcctccgatctacaaggcgacggaccaaca
gacgcaaggccaccggcgccgcagccgccgcatctgcgatcttgcccgcc
gccgctgccgccctgagagatggcgaaggcgtcttacgcggtggggttct
ggatccgggagaccggtcaggccctcgaccgcctcggctgccgcctccaa
gggaactacttcttccacgagcagatttcaaggcatcgcacacttatgaa
catctttgacaaagcccctcatgttcacaaagaggcatttgtcgctccaa
gtgcatcccttattggtgatgttgaagttggaaaagggtcttcaatttgg
tacggatgcgtcttaagaggtgatgcaaataacgttcgagttggatctg

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