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GrainGenes Sequence Report: CA022178

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Sequence
CA022178
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC162045
NCBI UniGene
Hv.16517
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001050937.1 Os03g0687000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HZ
Tissue
pericarp
Developmental Stage
0-7 DAP (days after pollination)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HZ42G02r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ42G02 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HZ42G02
Probe
baet31E1010
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 395 Std Error: 0.00; Plate: 42 row: G column: 2; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); pericarp 0-7 DAP(days after pollination). Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp
DNA
cggcacgaggctcaacaagggcaggctagacctcttctactggatgatcg
ccggcatcggcgtcttcaacttcgtctacttcatgatctgcgccaggtgg
tacaggttcaagggcaccagagacgcctgagctttgcacaaccatgcaat
agaataaggtcaggtgattaattggcgtgccttatactttgttgttgtaa
ttttttggttctggtgttgtgaaatcaatcaatctatagatgaagtgggt
ggggaaagaaaagaagaaagatagtaggcaacaatggctggttaatttct
tgtaagttgttaaaatgaaggaaaaaacaatgaggtggttaatttcttgt
aagttgtccaaatcatatgaaaggtaaatgaatgtctttcactgt

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