GrainGenes Sequence Report: CA021827

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Sequence

CA021827

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Cytosolic triosephosphate isomerase'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

pericarp

Developmental Stage

0-7 DAP (days after pollination)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HZ41D21r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ41D21 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HZ41D21

Probe

GBS497

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 331 Std Error: 0.00; Plate: 41 row: D column: 21; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); pericarp 0-7 DAP(days after pollination). Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp

DNA

cggcacgaggatcgccaccaaatccaatctcgatctcccgtccgcccgtc
ccaaccaacccaatccgacccgcctccgtcgccatgggccgcaagttctt
cgtcggcggcaactggaaatgcaatggaaccgtcgagcaggtagaggcga
tcgtccaaactctgaacgctggacagatcgtctctccggatgttgtcgag
gttgttgtcagcccgccctatgtcttccttcccatcgtcaaggctaagct
gcgcccagagatccaagttgctgctcagaactgctgggtgaagaagggcg
gtgctttcaccggtgaagtcagtgctgagat


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