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GrainGenes Sequence Report: CA018424

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Sequence
CA018424
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Hv.30837
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001061158.1 Os08g0189300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HV
Tissue
germinating seeds
Developmental Stage
germinating seeds (48-96 h)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HV08J09r HV Hordeum vulgare subsp. vulgare cDNA clone HV08J09 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HV08J09
Probe
kr63F0111
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 555 Std Error: 0.00; Plate: 8 row: J column: 9; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Roots were grown for two days on filter paper at room temperature. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
cggcacgaggcaatggcatcctccacctccttactctatcttcttaccgg
ccttcttgctctggcctcatggcacgccactgcatatgatcctagccctc
ttcaagacttctgcatcgctgatatgaagtccccagcacgagtaaatgga
tttgcgtgcaaggacccaatggctgcaacctcagacgacttcttcaaccc
ggccatgctggataaggctagggatacaaaggccagcaaggtcaggtcca
acgtcaccaacatcaatgtcataaatttccctggcctcaacaccctgggt
atctcgctagcacgcatcgattatggaccactaggggtgaacacgccaca
catacacccccgtgccactgaactcctgaccgtgcttgagggaacactct
accttgggtttgtcacatccaacccaaacaggctcttctccaagatagtt
acaaagggtgatgtattcgtattcccaaaagcaatgatccacttccaaat
gaacctagcacatgacaagccagcagccgcgttatcctcgctcagcagcc
aaaac

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