GrainGenes Sequence Report: CA017518

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Sequence

CA017518

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf176o18, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

germinating seeds

Developmental Stage

germinating seeds (48-96 h)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HV05H05r HV Hordeum vulgare subsp. vulgare cDNA clone HV05H05 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HV05H05

Probe

BaGS17D21

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 354 Std Error: 0.00; Plate: 5 row: H column: 5; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Roots were grown for two days on filter paper at room temperature. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb

DNA

cggcacgaggggaaggagaaggccaaggtctcgtatgacaggagaaagca
gctggcgaagctccgcgtcaaggccgagaaggcagccgaggagaagttgg
gaactcagctggagatcctggccccgatcaaatactaagagagatcagag
cttttgtgcgccttcagttttgggatacctctgtcctcctcttgtcttta
agtagtttggtaactctgttagtacaacctggttggtaaacctttgttat
gcatgcagttctgctaccctcatttgaaattttctacttggtcaactgtt
tcgtttatcaaatgtgtgcatttgaagattcttgtgaatgtctatcaggt
gatt


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