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GrainGenes Sequence Report: CA016966

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Sequence
CA016966
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC162003
NCBI UniGene
Hv.10887
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, strongly similar to NP_001066807.1 Os12g0497400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HV
Tissue
germinating seeds
Developmental Stage
germinating seeds (48-96 h)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HV05H02u HV Hordeum vulgare subsp. vulgare cDNA clone HV05H02 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HV05H02
Probe
GBS882
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 572 Std Error: 0.00; Plate: 5 row: H column: 2; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Roots were grown for two days on filter paper at room temperature. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
gcaaatttgtacgatgttacttgagaccaaaattattgcttgactccaga
acactaggataaaactggatcatcccctcacctaatgttttagagaattc
ttcaagacatttcagcagcggggagaagataacaacatacagtgagagac
aggcaaaaccccgattcaaccacagtacattacatctcatctggacaaca
tatatcttagcagtgatcataggtacagttggatgagctcatcgctcaca
acggatggtgtcagaacaccaaggtcagtcagaagaagtgtgaggtactg
gggaggagtgtagtctcttgccgatgtctcaacttccacaccagcaggta
tagggactccaaaatctatcggccgatgagctggtgtcatgtccttctgg
tccaggggatatagacgagcaaacttataactttcagcagccacatatag
aggtttattcatgctatgagctacaagagctatctgataagtccccatca
tattaataattcctccactttcaacaaccccatcagcaccaacaaatacc
atgtcaacttcatccatggagt

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