Query (optional)   in Class  

GrainGenes Sequence Report: CA016921

[Submit comment/correction]

Sequence
CA016921
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC155182
NCBI UniGene
Hv.29967
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, strongly similar to XP_002336980.1 tubulin, beta chain [Populus trichocarpa]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HV
Tissue
germinating seeds
Developmental Stage
germinating seeds (48-96 h)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HV05J03u HV Hordeum vulgare subsp. vulgare cDNA clone HV05J03 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HV05J03
Probe
BaGS33M05
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 498 Std Error: 0.00; Plate: 5 row: J column: 3; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Roots were grown for two days on filter paper at room temperature. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
gagactagtagcactactgaataacacaccaagcagtcatcagtcaaacc
ttacaccaatgctcatcacgacatgagcagaccaccaccacaagtccaca
taacgaaaaaataagtctgacaaggcaacacgaccgacgtttagaacggg
taaaagaatagcatctagctatcaccacctgacagcaccacgagcactcc
accaacttagcgagcgatataaggcatatcaatgggaagaacgggaggag
acgcgcctcagacggcctcctcgtcctcctgctcatcctcatagtcctct
tcctcctcgactgtcgcgtcctggtactgctggtactcggccaccaagtc
attcatgttgctctcggcctcggtgaactccatctcgtccatgccctcgc
ctgtgtaccagtgcaagaaggccttcctcctgaacatggcggtgaactgc
tcgctcacgcggcggaacatctcctggatcgacgtggagttgccgacg

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.