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GrainGenes Sequence Report: CA015800

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Sequence
CA015800
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC159996
NCBI UniGene
Hv.20006
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001131381.1 hypothetical protein LOC100192707 [Zea mays]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HV
Tissue
germinating seeds
Developmental Stage
germinating seeds (48-96 h)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HV11P02u HV Hordeum vulgare subsp. vulgare cDNA clone HV11P02 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HV11P02
Probe
kr15H0915
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 545 Std Error: 0.00; Plate: 11 row: P column: 2; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Roots were grown for two days on filter paper at room temperature. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
gacgccaaaagctaccagcttaattagcctacattgcttgcaaatagttt
cccacggcgaacttcagaccaatgaatttgtacaatgtgaacagtacaac
caaccatccaatcacagcctaacagaacgaaaaataaagacaaaagggac
atcataggcaccactgtacattgtgcgcgagaacgaggtctgaagcagcg
aagcatggctcgatagtctggcagaatccgcgacaccttaggaatggatg
caattcgctatcactggaaagcatgaatatgaagctagacgacagttcca
gaaaatatgcacatcacatgatcctttgggcattgttcctcaaaacaatg
cgcttaatcagatgtataaaagctgatagttgtttgccgcagaacttcag
aagtccggtcaaggctatgagatgccaccagatatgtgagggctgcatat
ctttcttggctaagatataaaggctttcctctgtgcatttcatgatcctc
ctcaccgaaggcatcaaggtacagagatggccaaaatgcaagacg

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