GrainGenes Sequence Report: CA014231
Sequence
CA014231
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC183242
NCBI UniGene
Hv.25215
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf120g05, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HT
Tissue
endosperm early
Developmental Stage
0-16 hours after imbibition
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HT10M04r HT Hordeum vulgare subsp. vulgare cDNA clone HT10M04 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HT10M04
Probe
kr30F1212
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 631 Std Error: 0.00; Plate: 10 row: M column: 4; Seq primer: M13rev. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgaggaggacttgaggtgcttatcttctcctccctgtagaaggag
gactgtataaattgctggcttctaccagagggccgcgtttggttcaaatt
gtcgtgtttgtggctctcttgtcgaagatgccttctaaggtgccccgggc
gccgcaagttcgttagctaggatgctataatgacatcccctttactgttg
cttagttccagcctcggtaatgttcttggagttggacatcctttgctcct
agtcctagatgctcgccgataattagcagaacagacagcgctcgaagaca
tcttgaataatttagatttcagcatccaggttctgtgcgaagatttcatg
ttcagttttgagtagacctcaagttggatatgagctgcatcaattctttc
gttggttggtatgcatgccccttgtgttggtgctctatactctgcccatg
atccaagcaattgcatggattccatcattgtttggttgtttatttgtttg
atccttaagggaatattgtgtgccatggtatttatttttgtacaaagtat
aactgctgaacctttgcaaaagtcgccgagtttggctctgtggttgagta
ttttattgaaaaagttgtggttcttttactg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CA014231
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||