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GrainGenes Sequence Report: CA009818

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Sequence
CA009818
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC177895
NCBI UniGene
Hv.4892
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf114b04, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HT
Tissue
endosperm early
Developmental Stage
0-16 hours after imbibition
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HT12L12u HT Hordeum vulgare subsp. vulgare cDNA clone HT12L12 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HT12L12
Probe
BaH32E20
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 542 Std Error: 0.00; Plate: 12 row: L column: 12; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
atcaaccgacagatccttcattgaaatccatcacatacatagcatgcaac
ttcttgccagtttcaagaatggcatgggagagcatgcaaccctccagcca
tttgccgctccatggccgcgtcagagtcctcatgatacaatagtcctact
actacaagattacatttccaggttccgagtttatatacacatcttcctcc
acgccgggaccaaacctccccgcaaccgcaagaggcacacggcgttcgcc
acaaccaggagcgcacgcacatcctcagtacgcatggctggtggcctgca
tggcgcttccggcacggtggcgttggaggccgcaggcacagtggacgtta
gtcaggctgaggcagccgctgatgatgagggagtgcctgtctgggcaggt
gtggagcgacgggaaggagtcgcagaccgcctgaaccgcgggaggcgtca
aggccgtgcactggctgatgttgaggtttgccagcccgtcgacgtccttc
gagctgctgctgctgctcctcatcgtgccccatctcccctcg

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