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GrainGenes Sequence Report: CA005775

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Sequence
CA005775
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC170631
NCBI UniGene
Hv.11661
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf112j09, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HU
Tissue
germinating seeds
Developmental Stage
germinating seeds, 16-48 h
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HU08M06u HU Hordeum vulgare subsp. vulgare cDNA clone HU08M06 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HU08M06
Probe
bast58C1206
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 553 Std Error: 0.00; Plate: 8 row: M column: 6; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); germinating seeds, 16-48h. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1.2 kb
DNA
aactgaacctcaaatcaaacaataaataatactactgtactaccacagct
ccataatagataaataaatagaataaaagaatgtttaggtgtggaggatg
ggcgggctgcagtagggcccgttgagccagccgtcggtgatgtgcttgta
cgcggcctcggtgaggtggatgccgtcccagctgaggtggtcggccgggc
tggcgcacgccaccgcgccctgcatcccgcacctcgcgctgtttgcgtag
ttgtacttgcctccccctgacccgcagcacgcctcgaaaaccgcgttgaa
tccgtatttgcttgggttctggaccatgtcgtagacggcggagtagaagt
cggcgtacatgacgcgcgccgcccggccgtagcgcttgcggaggcgggcg
atcttggcctgcagcaggttgttgtggaacgtgngagaggtcgttgaact
tgcggaggcagccgagggtgtcgtagtcggcgctgctgttggtgccgtan
acggtgaggtagatggggaagcagccgatggggagcaccccgggcaccac
cac

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