GrainGenes Sequence Report: CA005686

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Sequence

CA005686

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Transcribed locus'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

germinating seeds

Developmental Stage

germinating seeds, 16-48 h

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HU09E15u HU Hordeum vulgare subsp. vulgare cDNA clone HU09E15 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HU09E15

Probe

BaGS38M11

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 389 Std Error: 0.00; Plate: 9 row: E column: 15; Seq primer: M13uni.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); germinating seeds, 16-48h. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1.2 kb

DNA

ccgaatacaattcattatattagtcttcaaatacaattcatcatattagt
ctccgaatacaattcatcaataatgctagaacaatgtactatataaacac
tcttatatttcttccatagcgagagacaaatgagacaacaaacaacgagc
gagcaggccagagacaatttggcatgatatgcaacctgagagcgacaaaa
acaaagcatggattccagtggtgaactatgaggtgcggctcgtcaggaag
ggccgaagggctcggagatgcttttgctcctgctaccgcaagctttcggt
cctctatgggaggtcgaggcgccgcatcaagggccaacaataggacgcat
gtggagtccatgacaaccttagcttttaggctcttcagg


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