GrainGenes Sequence Report: CA000869

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Sequence

CA000869

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Cytosolic heat shock protein 90'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

embryo + scutellum

Developmental Stage

0-16 hours after imbibition

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HS06F20u HS Hordeum vulgare subsp. vulgare cDNA clone HS06F20 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HS06F20

Probe

bastl04A0101

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 422 Std Error: 0.00; Plate: 6 row: F column: 20; Seq primer: M13uni.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

DNA

agaagaaatatagctaacatatagtagaaccaacaataaccgaactgttc
cacgagtaccacaaataagctaaccgcgcttaatgtttggccagccaaaa
gacaaaaccgatacagctaagacgtgccaaaaccatccaacgacgacact
aagataaaagcaactagacggtgcagggcatggacatacgacgacgcgca
gccttggcgcttagtcgacctcctccatcttgctctcaccggcatcatcc
tccaggggcggcatgtcggtgtcgttctcaggcgcctcatcgtcctcatc
aatgctcaggccaagcttgagcatgcggtggatcctggtgccgaaggtgt
tggggtcctcaaggctgaagcccgaggtgagcagggaggtctcgaagaga
agcatcaccaggtccttcaccg


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