GrainGenes Sequence Report: BV693733

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Sequence

BV693733

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

AWW RFLP probe from cDNA

Data Source

genbank

Updated Nov 2006

Title

AWW11 AWW RFLP probe from cDNA Triticum aestivum STS cDNA, sequence tagged site.

Gene

cCAT

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Feature: STS: gene = 'cCAT'

Locus Comment: Contact: Thorsten Schnurbusch; ACPFG, Australian Centre for Plant Functional Genomics; University of Adelaide, Waite Campus; PMB1, Glen Osmond, SA 5064, Australia; Email: thorsten.schnurbusch@acpfg.com.au; Primer A: TTGAGCCGCGACGCCAGCTTCTGCC; Primer B: GAACAACAACAACGCCCTCACCGTC; STS size: 1383; RFLP probe; Template- 1-2 ul phage cDNA library, Chinese Spring,; from 5 and 9 days after pollination; PCR- Hot-start Immolase; (Bioline), AnnTm 60C, 35 cycles; cloned into pGEM-T easy.

Note: Vector: pGEM-T easy; RFLP probe

EMBL Feature

feature_gene	1	1383	put. wheat ortholog of O.sativa: gi\|54291140\|dbj\|BAD61813 Catalase	Location: 1..1383
STS	1	1383	Location: <1..>1383

DNA

ccggcttctgcccgagggacgtgtcacactgtgaccagtatgagacccag
atgccctggatttcatgggttacgcgagcatccgtgagcgcatcaaccca
ccgctgtaggaaacggtcttgcctggcagggtcgaaggatcggtatctct
cgccagcctgcttgaaattgttctccttctcgataatgcacttctcccgg
cagccagatagaacgcgaggagggatagggtactgttcagcatgacgagt
aggatcaaaccttgaagggaagtagttcacctcctcatccctgtgcatga
aattcattaagccatcatgatggttgttgtggtgagcacatttcggggcg
ttcacaggaagcatcaagtagtttggaccaagacggtgcctttgggtatc
agcataggagaaaatccttgtctggagcagcttatcatcagagtagtgga
ttccagggacagtgattgctgggcagaaagcaagctgttcattttctgcg
aagaaattatcaatgttcttgttgagcaccatccgtccaactggttgcaa
tgggatgatatcctctggccaggtcttggtgacatcaagagggtcaaagt
caaatttatcctcatgatcagcatcaatggtctgaatgaagagctttcca
ttcttgggtaattcccagctgcaatagaatcggtcaagtcctttgnggca
tgggtgtggcaggtgcctcctacagtaacggcttcatcatctaagaggca
cttcacaccacatgtaggcttccaatggaacttaacaaggtgcgcctttc
catccctgctgattaaggtgtaggtgttgacaccaaaaccgtccmwgtgc
ctgtagktgagtggaatgccaacattcatcaaagaggaagggtgaaacat
gtgcagactctcccggggkggtgcgaaaaggaagwcaaactatttctccc
agttctcctgcatgttggtctttggacttggcttgaaagcatggacccat
gtcagggaacttcatcccatctcggatgaaaaacacaggcatgttgttcc
caacaaggtcaaagttaccctctctggtgtagaacttcaccgcaaaacca
cgtggatccctcaaggtctcagggcttccangctcatgcacgacagtaga
gaaccggacaataaccggggtctgaaccccaggagcccggagaaagtcag
cacatgtgagctgagaaacatcatgagtcacctcaaagaatccctttgca
ctggcnccccgtgcatgaacaacacgttcagggatacgctcccggtcaaa
ctgcgcaagcttttcaatcagatggtaatcctcaaggaggataggtcctc
gctgcctgacggtgaggcgcgttgttgttgttc


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