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GrainGenes Sequence Report: BV211344

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Sequence	BV211344
External Databases	Data at GenBank Data at EMBL Data at DDBJ
DB Remark	Locus Source: Triticum aestivum (bread wheat)
Keyword	STS
Species	Triticum aestivum
Clone Library	Wheat var Chinese Spring Genomic DNA
Data Source	genbank Release 145, Dec 15 2004
Title	BARC324 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.
Probe	BARC324
Remark	DB_xref: taxon:4565 Feature: primer_bind: On complementary strand. Feature: source: mol_type = 'genomic DNA' Locus Comment: Synonyms: XBARC324; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: CCAATTCTGCCCATAGGTGA; Primer B: GAGGAAATAAGATTCAGCCAACTG; STS size: 247; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 52 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3. Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript
EMBL Feature	primer_bind 447 466 Location: 447..466 primer_bind 670 693 Location: complement(670..693) STS 447 693 Location: 447..693
DNA	atcaactccaacctaccctttattaagttttcccacaaatattattaagt gtcgcatctgtcaagatcatccaacaactgtaatggcatagtcgctcaaa gttacccataaccggggacacggctaatcatgattagttattaatactct gcagagtttagtactgctttacccactggacccaaccctgaagattgaga cgaagtctttcagaagcagtcccctagtcccaacaggaggcccatcccaa ctattatatctacatctgctggcacatccgggtaagggttccacaactga taaactatgccaaagcccacttagccagtggtcgcacatggaagctacta gtcactaagtttgtctaatctttttgagcctgggcgaccgtccacttaca ttcagaaggtataaaccatgatgttcttgaaaccaccctagcaataccaa ttctgcccataggtgaattaaatcctgagttactactcaaattattatta ttattattattattattattattattattattattattattattattatt attattatatataggcaaaagcaaacctatgcaacaatcttgtggaggta gcaatcatagccacatgttgagcttgggatctggtccaaaacttttccat ctttgaatttctcacatttcagttggctgaatcttatttcctcttataga tggttttggaccacgagcgcatgtgcctatgcactaggcccagcccatag cctgccaaccctgcgacatgccagtcatcggcccgcgtgaaacagttcca agcccatgaaaggttcctatatgcgcatggtgctcagcttgtgaaaggtt aattatttttttcgccttccttttttattagttttgcaccccctatccga taccctcactcatattcttattgttgggctgcttggaacccgtccaaatg ggcacatagcatgggaacgtcaacattatggccgttaagagtaaaacgaa tagttgagaagcccaatgcaccggccgatttgtgtagcaaggtgagtact atgcaaggtttctgtgtcaggaagaagaaagaaaacttggcatgtgtacc atggtagtgaagcacgagttttaaaagg

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