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GrainGenes Sequence Report: BU999880

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Sequence
BU999880
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC168913
NCBI UniGene
Hv.2440
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf42e02, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HS
Tissue
embryo + scutellum
Developmental Stage
0-16 hours after imbibition
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HS17B20u HS Hordeum vulgare subsp. vulgare cDNA clone HS17B20 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HS17B20
Probe
basd19k10
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 545 Std Error: 0.00; Plate: 17 row: B column: 20; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
agaggaaagccttttattaacaatcagtcaccattgtgtggaagattcaa
cttattttacatgtttctataaggattcgagatgggccaatcgcgaacat
attatcactttaaagagctctctatgtcgattacaaaccggtacctcaca
tcccggttgacaagcctctcgagagcctggttgatgtagtctatctttat
caactcaatgtctgggtagattttgtttgccgcacagaagtttatcatct
cctgtgtgtccttcgtgcctccggttacactcccagataaagtccgtgca
cccagattaagtgttgctggatgcactctgatttctccagggaagccaac
tagtgccatcaggccaccaactttcagaagcgcaagatacgggtcaaatg
ggtggtcaccagaggcagtatcgacaatgaagtccagggaacttttcagg
gactccatctgttttttatctgatgaaaccacaaaattatctgcaccaag
aaggttgattgcttcatctcttttcgattcacttgtactcaaaac

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