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GrainGenes Sequence Report: BU998847

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Sequence
BU998847
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC169618
NCBI UniGene
Hv.2895
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf140k04, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HI
Tissue
female inflorescences
Developmental Stage
female inflorescences (approx. 3 mm in size)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HI12F13r HI Hordeum vulgare subsp. vulgare cDNA clone HI12F13 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HI12F13
Probe
BaSD14M22
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 640 Std Error: 0.00; Plate: 12 row: F column: 13; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgagggttacctcggcattgantatatggaagggattgatggttg
cgacagggagcgagtcgccggttgtggtggttctttctggtagcatggag
cctggatttaaaaggggtgatattctgtttttgcacatgagccaagatcc
tatccgcactggagaaatagttgtttttaatattgatggccgtgaaattc
caattgttcaccgtgtgattaaggttcatgaacgccaagaaagtggagaa
gttgatatcctcacgaaaggtgataataattttggggatgaccgactttt
gtatgcacaagggcagctttggcttcagaagcatcacattatgggacggg
ctgtaggctatctaccatatgttgggtgggttactattgtgatgactgag
aagccgattattaagtaccttctgattggcgcgctgggcctgctggtcat
aacatcgaaagagtgaagctgtcaacctttgtgcatggtttgatcttgta
gtgcaaatgtaatcgagggcagaacaagttttgttgcgccgtgtaccaac
tggacgatgtatttttatctgttcctacagttgtctctgcccaaaagcct
aaacccagctcttatgcaacagtcatcttctgatggtctt

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