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GrainGenes Sequence Report: BU997420

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Sequence
BU997420
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC160828
NCBI UniGene
Hv.486
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001054001.1 Os04g0634700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HI
Tissue
female inflorescences
Developmental Stage
female inflorescences (approx. 3 mm in size)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HI07O20r HI Hordeum vulgare subsp. vulgare cDNA clone HI07O20 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HI07O20
Probe
BaH50I23
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 641 Std Error: 0.00; Plate: 7 row: O column: 20; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgaggacactcttgtcccgtggtagtcttcatcaactctagaagc
ggtggccaacttggaagtggtttgatcaaatcataccgtgaagttctcaa
tgaagcacaggtttttgatctctctgaagagtcccctgacaaggtgctgc
acaggttgtatgccaacttccaaaggctcaagaccaacggtgacactctt
gctattcaaattgagaagagcctgagactaattgttgctggtggtgatgg
cacggcaagttggctgcttggagtagttagtgatctgaagcttacgcact
caccccctgttgctactgtgcctctgggaaccggaaacaaccttcctttt
tcattcggatggggaaagaagaacccagcgactgaccaagaggcagtgaa
atcattccttgggcttgtgaaaggagcaagagaaatgagcattgatagtt
ggcatatcatcatgaggatgcgagttccacaggaaggtccatgtgatcct
attgctcccttagagctgcctcattcactgcatgcattccaccgtgtaac
aggctccgatgagctcaatgtggagggttaccacacattccgtggcggat
tttggaattactttagcatgggaatggatgctcaagtgtcg

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