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GrainGenes Sequence Report: BU996075

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Sequence
BU996075
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC188151
NCBI UniGene
Hv.23700
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Guanylyl cyclase'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HM
Tissue
male inflorescences
Developmental Stage
male inflorescences (approx. 2 mm in size), green anther stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HM12F16r HM Hordeum vulgare subsp. vulgare cDNA clone HM12F16 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HM12F16
Probe
baet45E0410
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 480 Std Error: 0.00; Plate: 12 row: F column: 16; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cgggcacgagggcacgttctaggaagcgtgaaatggtgcccatgaaaagc
ttagatgaggcccgcaaatcctttggaactgatgaggatattctcttggt
ctccttaactggaaagaacgggacgaagctgtcgcgtaaactgccggccg
gctctccatagactgctcaccagcctccagcctcacacctgtgtatactc
tgtacatgtcccctgtagttatgattaccgcttgtcaagtccggtatgta
gtttgagaacattcttacagccgataggaagatagaagaacccagcaagg
cctaagcttctgcaaacggcgcagcagcgacaccgtggagcacgaactgc
ttcgagcacaagtttcgtgccatcatggccgggcagagatgtagcatatg
tacagatacatccacgaataaaatggtggcctcgtggtgccatgtattcc
tacctgatttcaacactttctacctcgtcc

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