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GrainGenes Sequence Report: BU995475

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Sequence
BU995475
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC175741
NCBI UniGene
Hv.6759
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001048853.1 Os03g0130300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HM
Tissue
male inflorescences
Developmental Stage
male inflorescences (approx. 2 mm in size), green anther stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HM10F24r HM Hordeum vulgare subsp. vulgare cDNA clone HM10F24 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HM10F24
Probe
kr13F1012
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 479 Std Error: 0.00; Plate: 10 row: F column: 24; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
gaggaattcggacgaggcggacgtgcgagtcgcggagcccaggttcaggg
gcccctgcgtgcgcaggtccaactgcgccaacgtctgcaagaccgagggc
ttccccgacggcaagtgccgcgggttccgccgccgctgcttctgcaccac
ccactgccaccattaaccaacaggcctctgcttcgcgtcatgcatgccta
gcgcgcttcgtccgcccgtgcacgtacgacgacggtggctgaactgcgcc
tctctcttcctgcgtgcgtccatgcgtgtccatgaataagtctcagcttg
tctcggcaagcttcccttcttccttttttttgtatgtttgttacgagtgt
gcacgagtcgttgtgtctcgctcggttcgttctcagagtttgctgtaagg
tttgccaattatcacagtggaatccttctttcgatcatgtaagtttttct
tctttgaaaattgaaatcatgtgtggtgt

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