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GrainGenes Sequence Report: BU995194

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Sequence
BU995194
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC160142
NCBI UniGene
Hv.11603
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, strongly similar to NP_001046902.1 Os02g0502500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HM
Tissue
male inflorescences
Developmental Stage
male inflorescences (approx. 2 mm in size), green anther stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HM09I04r HM Hordeum vulgare subsp. vulgare cDNA clone HM09I04 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HM09I04
Probe
bastl38A0501
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 661 Std Error: 0.00; Plate: 9 row: I column: 4; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggttgaaggtgcacttcaatggggagagagacatcatggtcaagggaga
gttatacaacagaagttgggtgatgttgcagcagttttgaaagcatttgc
agattatgttgacagcctatgcgggaccccctaccctatggactatgaaa
tatggttgcgaaggttccgaagaacaataaatgaagatcatgggaaggag
gttgatacatcaagttagtactgcagcacatgttcttacaaaattgcaac
atggaagatgaagaacagtgtttcataccttgtattgttgttgtagataa
tttatctgtagagttgtaggtcttgtgaaaggtccatatagctcatagta
cccttctcggtcttgatccatttcacccgacgggtacttgaaaacgtcca
atctggtgacctgctgctgatctcaacttggatttctgactgtattaccc
tcccaagaatgcagtggagatccttgaggatatctgcaacaggatgtgct
ctggaaattctctcctccaagttggtgagctgcgctttggctgatgtcat
cttgattagtaccttgaatgttgaaacttataagtggtaggcaaatgtgg
atctcaaagtgtgtggtatagtcggcaaatttttgctcacttttcttttt
gaaattgcacc

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