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GrainGenes Sequence Report: BU994143

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Sequence
BU994143
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Hv.24061
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf90d11, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HM
Tissue
male inflorescences
Developmental Stage
male inflorescences (approx. 2 mm in size), green anther stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HM05O08r HM Hordeum vulgare subsp. vulgare cDNA clone HM05O08 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HM05O08
Probe
BaGS32P17
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 535 Std Error: 0.00; Plate: 5 row: O column: 8; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cgtcttcctcgccgccgcgccaccgtcgttagccatggcgccgaatattt
caaggcccctgcggcttcgtcgaagccggcaaagtcgggcggagggaagc
agaagaagaagaagtggagcaagggaaaacatattgagaaggtgaacaac
gcggtgctcttcgaccaggccacttacttcaatcntttcaccgaggtgcc
cattttcaagcagatcacgccatccgtcctctccgagcgactcaggatca
atggctctctggctcgcagggctatcaaggacctgatggagagggggctc
attaggatggtgtctatccactccagccagcagatcttcaccagggccac
aaacacctgatttatcattatcatgtgttctatggctgtgttctctagtc
cagtagactgaggtgttgctgttatattcaagttcactatgtccagggag
gcacttgtgccaccagtaatgttgaattgcttttgagttttggtacactt
tatgatcgttttgtcagcgcaatgaaatctacctt

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