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GrainGenes Sequence Report: BU993521

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Sequence
BU993521
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC165623
NCBI UniGene
Hv.29528
DB Remark
Locus Source: Hordeum vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001131696.1 hypothetical protein LOC100193057 [Zea mays]'
Keyword
EST
Species
Hordeum vulgare
Cultivar
Golden Promise
Clone Library
HD
Tissue
callus
Developmental Stage
callus (5-10 mm in diameter)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HD13M22r HD Hordeum vulgare cDNA clone HD13M22 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
Clone
HD13M22
Probe
GBS767
Remark
DB_xref: taxon:4513
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 675 Std Error: 0.00; Plate: 13 row: M column: 22; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
cggcacgaggtatgaagctatttgtgggacagtacttgtagagtagatgg
gggagaaggaaagggaagcaagtttatttaggaagttatgatactgagca
aaaagctgccagggcatatgatgttgcagctcttaaatactggggactaa
atacaaagctgaacttttcgatttcagaatatgaaaaggaactggcggac
atacaagacatgtctccagaggaatgtgtgacatacttgcgaaggaggag
cagctgcttctcaagaggggcttctatttacagaggagttacaaggaggc
agaaagatggtcgatggcaggcccgcataggactgattgctggaactaga
gacatttaccttggaactttcaaaaccgaggaagaagctgcagaagcata
tgatattgctgccattgagatacgtgggaaaaacgcggtgaccaactttg
accgaagcaactacatggacaggggcatgcattgtatagaaggggcaggg
ttgaagctgcttgcaacgaagccagaatgatttgatatcgtatattgaac
agatttggtaagccatattttggggcttagtggtacatacaagatagaag
aactggtcgcagcctgtcagtatttgctgctgtatgattcttcagattat
atatagttcttccagagagaatttc

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