GrainGenes Sequence Report: BU992160

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Sequence

BU992160

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare

UniGene title 'Transcribed locus, moderately similar to NP_196976.2 protein kinase family protein [Arabidopsis thaliana]'

Keyword

EST

Species

Hordeum vulgare

Cultivar

Golden Promise

Clone Library

Tissue

callus

Developmental Stage

callus (5-10 mm in diameter)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HD08P21r HD Hordeum vulgare cDNA clone HD08P21 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

Clone

HD08P21

Probe

BaAK14F01

Remark

DB_xref: taxon:4513

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 356 Std Error: 0.00; Plate: 8 row: P column: 21; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb

DNA

atctggatcgcttgctagagaaaagggattggagtcatatgtcgtccaac
tacagcgaagtgtcacagaactcgctgaggaagtgcagagattaaagctg
agaaataatcagcttgagcatcagatcaatgggttgtcgaaaaaagacga
aaggttgcgaagagagcatagtacgaagcaatgataatgatatatttatt
ccctgtagtccccgttgtataattcagctccccaattttgaacctggtta
gcgaaattaattgtattacgtttcggaatcaggtaggtgtatcaattgta
attgtaagtatattagcgagttcattcacattatagtatacatttttgat
ttgtac


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