Query (optional)   in Class  

GrainGenes Sequence Report: BU991896

[Submit comment/correction]

Sequence
BU991896
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC167017
NCBI UniGene
Hv.21515
DB Remark
Locus Source: Hordeum vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001044089.1 Os01g0720400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare
Cultivar
Golden Promise
Clone Library
HD
Tissue
callus
Developmental Stage
callus (5-10 mm in diameter)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HD08E01r HD Hordeum vulgare cDNA clone HD08E01 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
Clone
HD08E01
Probe
bast52G0414
Remark
DB_xref: taxon:4513
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 561 Std Error: 0.00; Plate: 8 row: E column: 1; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
cggcacgaggcttccccgtgtgggacctcatctgcgagaacccgggattg
ctccaggccgaggtgcacccatggagcgacggcaaggacatggaggagac
gctgctgcggctcatcagccgggtgctcgtcgaggagagccagctgctgc
cgctcgactgcaagctcgagtcgctgccggtggccgtccaggacggcatg
cccatgccgctcggcgtcaagaactgatccatccatccatccatccgtgc
tgcattacgattaatttggggattctgtatctgatatatcttctgattct
gatttgcttgcctgattagctggtgtgtacgagtttgggtaggccgtcgc
aggaaactaattaagggtgaggtgttggagcgccaatggcgaactcgata
ataatgtatgaggcttgcagtattagctagtgtagcagtattattgacta
atttgtgagtagagtatcttcgttgggccgcctggcaatggtgaaggctc
gctgtatcccatctatctatggattgacaaaaagaataagtacatgagtt
gatctctgacg

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.