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GrainGenes Sequence Report: BU991467

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Sequence
BU991467
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC173600
NCBI UniGene
Hv.5551
DB Remark
Locus Source: Hordeum vulgare
UniGene title 'CDNA clone: FLbaf59e13, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare
Cultivar
Golden Promise
Clone Library
HD
Tissue
callus
Developmental Stage
callus (5-10 mm in diameter)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HD07A05r HD Hordeum vulgare cDNA clone HD07A05 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
Clone
HD07A05
Probe
BaSD17P01
Remark
DB_xref: taxon:4513
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 606 Std Error: 0.00; Plate: 7 row: A column: 5; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
ccttctgcaagatgataccaatgactcgctggagtggcaccatcttgttg
ctagatacaggcagatgaatcgcagagcagtagtagtgagtgcacttctg
gagcctgtacattacatgcatgatgtgggtgtcatctcagacgatgatca
tgaagatgatgcaggtggcatctcagacgatgatcatgaagatgatgtag
gtggcatgtcagacgatgattatcgtgagtagatctgcatgctggactgc
acctacaactgtgccatggttttactcattgtagtttagtgaacacctct
gaactctgcaagccttcgttgcaaacatttctgaagctgattctggaacc
cagaggacctgatctgagacaacttgctcttatggtttgcaactctgctc
aagcctggtcgtgttttctcttgtgttttcggtttagtggtgaatattgg
tcatacattacttttcaaataaatgccatttgtggacggattgcagaaat
cttttggcacactatgaaagcatgacactgtagacttgctgtatatatgc
tgaatgcaatctaatctttgtggagttgcatctccagggttgagcgattg
cgcttt

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