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GrainGenes Sequence Report: BU990978

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Sequence
BU990978
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC167014
NCBI UniGene
Hv.3154
DB Remark
Locus Source: Hordeum vulgare
UniGene title 'CDNA clone: FLbaf102m02, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare
Cultivar
Golden Promise
Clone Library
HD
Tissue
callus
Developmental Stage
callus (5-10 mm in diameter)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HD05J21r HD Hordeum vulgare cDNA clone HD05J21 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
Clone
HD05J21
Probe
GBS885
Remark
DB_xref: taxon:4513
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 560 Std Error: 0.00; Plate: 5 row: J column: 21; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
atatccttgttgggagctactgtgcattctctaacaggggtggactggta
tggtgtttctgcattctctaacctgtttctgcaaaaaatcttcaaaatct
taaatgtacaattcctttgtggtacaggtccatcctcacacatccattga
agatcttgatgagctgtccacactcctccaagtccctcttgtcgctggaa
ccgtgaaccgtggtagcgaggtcattgctgcgggcatgacggtgaacgac
tggactgccttctgtggctcagacacaaccgctaccgagctctccgtcat
cgagagcgtcttcaagttgagagaaggccagcccacggcgatcgtcgatg
acatgaggaagtcgctgattgacagctacgtctaaatgatcaaagggcat
gggatgtggttcttgaacggtacttttggttgacagtagcagaaaaacat
gcctgtctaagctcttacttctggtgtaccttcgtcttccgaatttggaa
ttacaaacttgtgctttgtcctggtaaagcttacggtatttgaaggtgtg
cacttacgct

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