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GrainGenes Sequence Report: BU990814

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Sequence
BU990814
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC174161
NCBI UniGene
Hv.15753
DB Remark
Locus Source: Hordeum vulgare
UniGene title 'Transcribed locus, moderately similar to NP_173122.1 vacuolar-type H+-translocating inorganic pyrophosphatase, putative [Arabidopsis thaliana]'
Keyword
EST
Species
Hordeum vulgare
Cultivar
Golden Promise
Clone Library
HD
Tissue
callus
Developmental Stage
callus (5-10 mm in diameter)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HD05C09r HD Hordeum vulgare cDNA clone HD05C09 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
Clone
HD05C09
Probe
bah25l03
Remark
DB_xref: taxon:4513
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 473 Std Error: 0.00; Plate: 5 row: C column: 9; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
gtttgcgaccgttactggtatccttatggcgctcttcttgaacacctctg
gtggtgcttgggataatgctaagaagtacatcgagactggtgcacttggt
ggtaaaggcagtgaggctcacaaggctgcaattactggtgatacggttgg
agatccattcaaagacacggcggggccttcgatccatgttctcatcaaga
tgctcgccacgatcacactggtcatggctccgatctttttgtaatcatcg
gtcgagctcgcagttaaccccgcagagaaatatctatggttattgtagat
gaggcattaacgtgaatgggatgtaatgattcgcttttagtattgttttg
ccacattgaaaggagaggttcagtgctgcccattttgatggtgcccgtat
tttaccgttgctatttggacaaaaggatgcagacgttggagaatatatat
gctgtgacagagattagaatggn

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