GrainGenes Sequence Report: BU987229

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Sequence

BU987229

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Transcribed locus, moderately similar to NP_001053776.1 Os04g0602800 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

caryopsis

Developmental Stage

developing caryopsis, 16-25 DAF (days after flowering)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HF14C17r HF Hordeum vulgare subsp. vulgare cDNA clone HF14C17 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HF14C17

Probe

bastl55F0812

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 433 Std Error: 0.00; Plate: 14 row: C column: 17; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis , 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp

DNA

cggcacgagggagccaccggggtcgccatggacatcaatttcttcaacca
agatacaaccagccaaccaacagtcacatgcggccaccgatggggagtca
ccaaagtgggcagcctgcatggcctccctatggcatgggggagggagtgc
cctggggaggaacaggcgtgcatccatttacgacgggcgggctaccctcg
tcccttcccagaaaagattatgggagcatattttagtgtggactcggagg
aatgccattcattgccgggataaattatttgtactggcgaaaccggagtt
gcgatggttctggctattgattttccttttctttcctgcaatgttgctgc
atttgcgttgctccattgggtcctcgattgtcaagacactctcgaactct
atgatatataatagaatcgatggttacatcatt


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