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GrainGenes Sequence Report: BU978220

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Sequence
BU978220
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC169930
NCBI UniGene
Hv.2336
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, weakly similar to NP_001049481.1 Os03g0234600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HA
Tissue
embryosac
Developmental Stage
0-7 DAP (days after pollination)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HA12M13u HA Hordeum vulgare subsp. vulgare cDNA clone HA12M13 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HA12M13
Probe
kr65H0816
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 430 Std Error: 0.00; Plate: 12 row: M column: 13; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); 0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb
DNA
gatgcaaagtttaatgttgattatttggcagctggaacaaattctaaaac
agagtaacagtgcaacatctggaattgtctccgcttctacagattctgta
acttaacatgtgacaacacaaaagcaacatggaagccatggtacttccaa
tctcatcagttggtttgtatataggtccatcatggctcgctaggtggggg
tggtggtggaggcaaaagaagtgcaagacgctgcttgtatctctcgatcc
ggacttgccgctccttgcgcaattgggcacgtatcttttttgcacgagtt
acatgctcgctcaacaagtcagacgcagttgtaatagtatcattattggt
caatgctctgtccatcacttctctgatgcacagggtgttcctttcttggg
catatctctgctcttcttctgaaacttgat

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