Query (optional)   in Class  

GrainGenes Sequence Report: BU977117

[Submit comment/correction]

Sequence
BU977117
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC154062
NCBI UniGene
Hv.15148
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Ubiquitin polyprecursor fragment'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HA
Tissue
embryosac
Developmental Stage
0-7 DAP (days after pollination)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HA10N22u HA Hordeum vulgare subsp. vulgare cDNA clone HA10N22 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HA10N22
Probe
BaAK41K07
BaSD25N02
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 513 Std Error: 0.00; Plate: 10 row: N column: 22; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); 0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb
DNA
ggatattaacggctcacttattaatcatagatcatcatccaaagccacgg
cacaagttttagacacaaccgaacaggtccgtaatggaaacaacgacaca
accagacatgctccaacctttggtgagaacaccgacaacacaagacctgt
gaaccctgagacagaagcaacgtcatgaccaggcacttacttgccaccac
gcaggcgaagcaccaggtgaagggtagactccttctggatgttgtagtct
gccagagtgcggccatcctcgagctgcttgccggcgaaaatgaggcgctg
ctggtccggagggatgccctccttgtcctggatcttcgccttgacgttgt
cgatggtgtcagaggactccacctccagtgtgatggtcttgccggtgagg
gtcttcacgaaaatctgcatgcctccacgaagcctgagcaccaggtggag
agtggactccttctggatgttatagtcagccagggtgcggccatcctcga
gctgcttgccagc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.