GrainGenes Sequence Report: BU977032
Sequence
BU977032
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC157366
NCBI UniGene
Hv.3476
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf163e08, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HA
Tissue
embryosac
Developmental Stage
0-7 DAP (days after pollination)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HA10L15u HA Hordeum vulgare subsp. vulgare cDNA clone HA10L15 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HA10L15
Probe
bah60e11
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 613 Std Error: 0.00; Plate: 10 row: L column: 15; Seq primer: M13rev. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); 0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb
DNA
cagccacaaagtgggacacgacagcattttcattgtatgaagcaaatcca
tcaaaacagaatgcacaaacagcaagatttacagacccataaatgaaatg
gttcaacgaatctcaagtgttacatttatttatagctaggtattgcctaa
aaatgcaaggcacaagtgatgccagaatactctctggtctagattcccta
gtagatactactccctccgtcccataaaagtcccataaaataagagcgtt
agtgtcagaagctctcttatattacaggacggggggagcacttggcacac
gttttcaagtttaaaaaacagcacttcctgcaagcaaataacaaaaaggc
tgggacactttacattttaaccagcaaggatctccctttacaatgttcca
ctgagcaacaaatctggatattcgtctcgccaaccccaaagaaaattagt
tggcagacttcaacgagttcgaccataaggttcagtcctcaaacctgctt
atcttgtcagtggaatcagcattgctgaattcacgataatcccctgtttc
tatggctagttgcaacccctgtatgccaattgttgaaaatcgcttcgctg
atggtttgatgcc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BU977032
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||