GrainGenes Sequence Report: BU101092

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Sequence

BU101092

Contig

NSFT03P2_Contig13087

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001065790.1 Os11g0155600 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Chinese Spring aluminum-stressed root tip cDNA library

Tissue

Root tip at 1.0 to 1.5 mm stage

Developmental Stage

Seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE3361_E08_J15ZS Chinese Spring aluminum-stressed root tip cDNA library Triticum aestivum cDNA clone WHE3361_E08_J15, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE3361_E08_J15

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown for four days under hydroponic conditions with 1ppm aluminum, root tips were excised and snap frozen, and total RNA was prepared at University of Missouri in the JP Gustafson lab (Ross). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Chin) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

agcagaattctggatggctgccaatgtccaaaatcctcatgttagtacac
tgaagttaatttctgtgtacgttggtattggagtttgcacaatgatcttt
gtgctatcgagatctttatttgtcgttgttcttggcatccaaacttcaag
atccttattttcccagttactcaattcattgttccgtgcacctatgtcct
tctttgattctactcctcaaggaaggattcttagccgggtctcttcagat
ttgagtatcgttgaccttgatattccatttgcattcatgtttagcctcag
ttccagcttaaacgcatatagcaatgtgggggtattggctgttgttgtat
ggcaagttctctttgtagcactgccgatgatagttttggtaattcagttg
cagaggtactatttagcctcagctaaggaattgatgcggatcaacggaac
caccaagtctgctctagcaaatcatttaggtgaatcgatctcaggggcta
taacaatacgtgcctttgaggaggaagatcgtttctttgccaaaaatctg
gagcttgttgacaagaatgccggtccatacttctttaattttgcagcaac
tgaatggttaattgaacgtctggaaataatgggtgctgtggttctttctt
cttctgcctttgtcatggctcttcttcctgcaggaacttttagccctg


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