Query (optional)   in Class  

GrainGenes Sequence Report: BU100238

[Submit comment/correction]

Sequence
BU100238
Contig
Ta.9136.1.S1_at
Ta.9136.3.S1_a_at
NSFT03P2_Contig14264
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC314871
NCBI UniGene
Ta.54919
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001062518.1 Os08g0562200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Chinese Spring wheat drought stressed root cDNA library
Tissue
root
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE3316_H04_P08ZS Chinese Spring wheat drought stressed root cDNA library Triticum aestivum cDNA clone WHE3316_H04_P08, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3316_H04_P08
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under normal conditions, then drought stressed to 80%, 70% and 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA was prepared separately for roots collected at the three different drought conditions. Equal amounts of total RNA were pooled from all three samples, poly(A) RNA were purified, one cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab at the University of California, Riverside (Fenton, Turuspekov). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gaggggcaatgattccactatctggccttctgatggttgggcctggaaaa
cagctttccccttggatgctgtaaacaggtccaacaatgaggttcctctg
atcactggtgatcagcctttccagccagatgagttggcccagttgttgca
gacactacaagatgactcctcccagttgggctcaactatgaccgatctcc
cacactcttctattacaaattcagtcaagccagaagatgattctcttgtg
tattttgatgcaccctttgataactcaatgttcagtgatggatttagaca
gacgaatgaattccttggctccccagcaaccattctatctggtatcgaga
cactggatgatggtattccatactatgatgcaatggacgataacttattt
aatgatatgatgtgctctgtacagcagtcggctgggagcagctcccatgt
ctttaatgggccagttcttactcaagaggtcaacaatcccagctatacat
atagtccaactcaaaaggttgtagaacctaattttgtagttggggccccg
tcgtccgctaggttatctgaagctggtggccagttaaattgtgttgtttt
accagattctcaggctaagaatggctccatgggaaagcgttttgtaaaga
tgctggattcaatctcggctccccctgcttttgcagcagagttcccaggc
aaatccttgtct

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.