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GrainGenes Sequence Report: BU100188

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Sequence
BU100188
Contig
Ta.7168.1.S1_at
Ta.7168.2.S1_a_at
NSFT03P2_Contig18231
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC347115
NCBI UniGene
Ta.7168
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001062057.1 Os08g0480100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Chinese Spring wheat drought stressed root cDNA library
Tissue
root
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE3316_C06_F12ZS Chinese Spring wheat drought stressed root cDNA library Triticum aestivum cDNA clone WHE3316_C06_F12, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3316_C06_F12
Probe
MAG913
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under normal conditions, then drought stressed to 80%, 70% and 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA was prepared separately for roots collected at the three different drought conditions. Equal amounts of total RNA were pooled from all three samples, poly(A) RNA were purified, one cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab at the University of California, Riverside (Fenton, Turuspekov). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
agcccgccaacccctcctccgccggtgccggccgtggccccgccggcaag
aagctcggccccaatgctcgcgctggcggcggcggcggtgccgggaagaa
gaactctggctctgggaaggacgactccgacggcagcgattcggggaagg
agcagaacggtggtttcactgctaacggtggtagcgtcgccaagggcggg
cacgagaatggtgctgtcaaggacacttctcgcaccgcccgggctgttgt
tgttgacaatgacggcaaggagaacggggaccccaacgacggggccttcg
atgtaaataccttgcgtaggaagacaggaggaaagaagggcggcggcggc
ggcggcaagaagaacgatgtgaagaagccggccaagaccgagcccaagaa
ggttgtcaagcagatgagggtctgggacgacaaaccgccgaagaacgaga
agctggacttcacggacccggctgacgagagaggggaggaggtgattgag
aaggtgcttaccaaccagggggacagcatgatggataaggatgaag

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