GrainGenes Sequence Report: BU099395
Sequence
BU099395
Contig
Ta.5140.1.S1_at NSFT03P2_Contig14400
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC277153
NCBI UniGene
Ta.5140
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Multiple inositol polyphosphate phosphatase PhyIIc'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Chinese Spring wheat drought stressed root cDNA library
Tissue
root
Developmental Stage
Full tillering stage
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE3306_H09_O18ZS Chinese Spring wheat drought stressed root cDNA library Triticum aestivum cDNA clone WHE3306_H09_O18, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3306_H09_O18
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under normal conditions, then drought stressed to 80%, 70% and 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA was prepared separately for roots collected at the three different drought conditions. Equal amounts of total RNA were pooled from all three samples, poly(A) RNA were purified, one cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab at the University of California, Riverside (Fenton, Turuspekov). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gaggcctctccaccgtcaccaggtacgatgtgtccagggcgtccaacagc
gtggtctccgcgccggctatctccgacgagtgccgcgtgatccacctcaa
tctcgtcgcaagacatgggactcgtgctcctaccaagaagagaatcaagg
agttggataggctggctgtgcggttggggactttagtggatgaggcaaaa
caaggacctgacactgcttctctgaagaaaattccatcatggatgaaggg
gtgggagtcacgttggaagggtagggtcaaagggggtgaactggttagcg
aaggtgaggaagagctgttcaattttgccaaccgagtgaaggagaggttt
caagacctgtttgatgaggaatatcatccagacgtgtactcaataagagc
aacccaggttcctcgagcatcagctagtgcagtagcatttggtttggggc
tactttctgggaaaggaaagcttggagcaggaaataaccgtgccttctct
gttctgagcgagagtcgtgcaagtgatatttgtctgcgattctttgatag
ctgtaaaacatacaaggactacaggaaaagaaaggaacctgatgttgaca
agcaaaaggaaccaattctagaacatgtcacatctgctttagtcagccgt
tatcacctcaagtttacaacacaggatgtttcttccctctggttcctctg
caaacaggaagcatctttactgaatattaccaatcaagcttgtc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BU099395
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