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GrainGenes Sequence Report: BU099287

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Sequence	BU099287
Contig	NSFT03P2_Contig15067
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC298151
NCBI UniGene	Ta.7803
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001050053.1 Os03g0338000 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Chinese Spring wheat drought stressed root cDNA library
Tissue	root
Developmental Stage	Full tillering stage
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE3305_G04_M07ZS Chinese Spring wheat drought stressed root cDNA library Triticum aestivum cDNA clone WHE3305_G04_M07, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE3305_G04_M07
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under normal conditions, then drought stressed to 80%, 70% and 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA was prepared separately for roots collected at the three different drought conditions. Equal amounts of total RNA were pooled from all three samples, poly(A) RNA were purified, one cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab at the University of California, Riverside (Fenton, Turuspekov). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	actcaagggaagtgaatcacaccgggtgatcgtaacatattccattcagg tgggcagtagagatgctgggggctaggagatcgttctctaggctcgccgc ggcggttaggacgccggtggacgtgccgagaatgccggcgttcgaccacg tgccgctgccctacgacgggccgagcgccgtcgagatcgccaggaagcgc gccgagttcctcagcccctccctgttccatttctactccaagcccctcaa cattgtggaggggaagaaacagtacctctacgacgagcacgggcggcgct acctggacgcgttcgccggcatcgccaccgtgtgctgcggccacacccac cccgaaatcatcgacgccatcaccgcgcaggccaacctcctgcagcactc caccgtgctctacctcaaccacgccatcgcagacttcgccgaggcgctgg ccgccaagatgcccggcgatctcaaggtcgtcttcttcaccaactccggc acggaggccaacgaactggccatgatgatggctcggctgtacaccggctc ccacgatatcatatctctccgcaactcctaccacggcaacgcctccggca ccatgggtg

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