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GrainGenes Sequence Report: NSFT03P2_Contig13996

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Sequence
BQ842771
Contig
NSFT03P2_Contig13996
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Ta.56447
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001048445.1 Os02g0805900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Brevor
Clone Library
Wheat dormant embryo cDNA library
Tissue
Seed embryo
Developmental Stage
Mature seed
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2998_C02_F04ZS Wheat dormant embryo cDNA library Triticum aestivum cDNA clone WHE2998_C02_F04, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2998_C02_F04
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tggcgatggcgggcggcgggggactgaaccggtcatcgtcgcgggggcag
ctgccgccgcaggagctgctcgacgatctctgcagccggttcgtgctgaa
cgtgcccaaggaggacctggagtcgttcgagcggattctgttcctgctgg
agcaggcgcactggttctacgaggacaactccgtggagcacaacccctcc
ctcaagtccctctccttcaaggacttcacctccctcagtaagataaagat
cgctcctttcccctttaaatctgctcctgtctcgtcacggcccgggactc
cgagccccgtcgaggacgatggttcttggcggattcgtcgctgactccga
cattgtcgccgtcgcggtcgccgtcgtccttgtgtgcgcagtgttcaaca
gctgcgccgccctcaggccctaccgcgcgcacctcgacgacatctacaag
gacttcacccactacaagt

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