GrainGenes Sequence Report: BQ842458

[Submit comment/correction]

Sequence

BQ842458

Contig

NSFT03P2_Contig5364

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

EST

Species

Triticum aestivum

Cultivar

Brevor

Clone Library

Wheat dormant embryo cDNA library

Tissue

Seed embryo

Developmental Stage

Mature seed

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE2993_C06_F11ZS Wheat dormant embryo cDNA library Triticum aestivum cDNA clone WHE2993_C06_F11, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE2993_C06_F11

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

agcagccaaggcggtaagcagaaagtggagctaaaggtttcaaagatcat
tgtgattggggagagtgatcccacatcttttcctatacagaagaaacgag
catcaagagaatttctgaggactgtggcacatctccgtcctcggacaaac
acttttggtgcagtggcaagagtaagaaatgctctggcatatgcaactca
taaattttttcaagacagtggatttatttgggtatcaagccctattatta
ctgcctcagattgtgaaggagcaggggagcagttctatgtcactactttg
ctttcaaatagtgctgaaggaggctgcctagtcaacagcattccttcgaa
ggatggaagggtcgattggtcacaggatttcttctgcaaaccagcatttc
taacggtgtctggacaactgaatggcgaaacatatgcttcagctctatct
gatatttacacatttggtcccacatttagggctgaaaattcaaacacttc
aaggcatttggctgaattttggatgat


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.