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GrainGenes Sequence Report: NSFT03P2_Contig10595

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Sequence
BQ842273
Contig
Ta.10748.1.S1_at
NSFT03P2_Contig10595
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC335088
NCBI UniGene
Ta.10748
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001054927.1 Os05g0214400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Brevor
Clone Library
Wheat dormant embryo cDNA library
Tissue
Seed embryo
Developmental Stage
Mature seed
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2991_A05_A09ZS Wheat dormant embryo cDNA library Triticum aestivum cDNA clone WHE2991_A05_A09, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2991_A05_A09
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ccacttccgcctccgccccttcggactaaaccgtctcagcggcaaccggt
ggtcgtcttcgcccacggcgccggcgccccctcctcctctgattggatga
cccgctggaaggagatggtgaaagatgccctggatgccgttgaagtggtg
acattcgactatccatatatgtcaggtgctaaacggaggcctcccccaaa
ggcggacaagctcgtcgaccaccatctcagtgtggtgaaggatgctgtag
cagagcatcctggtcacccgcttgttcttatggggaagtctatgggttcg
agggtcagctgcatggtagccagctccgatgatatcagtgtttttgcagt
catttgcttgggttatccactgaagggagtgaaaggggcactgagagacg
agatactactgaagctgatggttccaacaatgtttgtgcagggcaacaaa
gatggcctatgccccttggacaagcttgagttaactcggaagaagatgac
ctgcaagaatgaactgcatgttgttgatggtggtgaccactctttcaaag
t

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