GrainGenes Sequence Report: BQ840609
Sequence
BQ840609
Contig
NSFT03P2_Contig12209
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Aegilops speltoides
Keyword
EST
Species
Aegilops speltoides
Cultivar
F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1 ) F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1)
Clone Library
Aegilops speltoides pre-meiotic anther cDNA library
Tissue
Anther
Developmental Stage
Pre-meiotic anthers
Data Source
genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title
WHE4201_E04_I07ZS Aegilops speltoides pre-meiotic anther cDNA library Aegilops speltoides cDNA clone WHE4201_E04_I07, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE4201_E04_I07
Remark
DB_xref: taxon:4573 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov in Devorak's lab). Pre-meiotic anthers were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made , and the cDNA clones were in vivo excised to give pBluescript phagemids at the University of California, Davis (Akhunov in Devorak's lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov in Devorak's lab). Pre-meiotic anthers were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids at the University of California, Davis (Akhunov in Devorak's lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tcggcacgaggatcccgactcgctgcaacttgccagccacctcaccggcg
gttcattactgcatacgtcctgctcgacgtaatgctcgtgagagcctttt
gcgaccggtgagctaactacccagcagcttttcttgggccgggtcatggt
ggattgattgagcgtccacggcgatgggcggctcggagaggaccgttgtg
tggttcaggagggacctcaggatcgacgacaacccggctctggccgccgc
cgccagggacggcgcggtgctccctgtcttcatatggtgcccggccgagg
agggccgcttctaccccggccgctgctcccggtggtggctcaaggagtcg
cttgcccaccttgccaggtcgctccaggcgctcggctgcccgctcgtgct
catccgagctcacactactcttgccgcgcttctgcagtgcgttggttcca
tcggcgccaccagagtggtctataatcatctatacgaccctatttcactt
gtgcgggatgacaaaatcaagaatgagcttttgggccttggaatttctat
gcagagtttcaatggtgatctgctatatgagccatgggaagtttatgatg
agaatgggcttgcatttacaacgttcaataagtattgggaaaagtgcatg
aaattgcatatcgagatatcaccatctcttgcccccttggaggttggtgc
ctgtctcaggtatagaaaacatttgcagtagttcaattgataacctaggt
cttgaatcaagtaaggatgaggaatcaagcaatgctttgctaagcagggc
t
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ840609
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||