GrainGenes Sequence Report: BQ838703
Sequence
BQ838703
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC368213
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Butte 86
Clone Library
Wheat developing grains cDNA library
Tissue
whole grains
Developmental Stage
3-44 days post anthesis seed
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE3591_G02_M03ZS Wheat developing grains cDNA library Triticum aestivum cDNA clone WHE3591_G02_M03, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3591_G02_M03
Probe
MAG547
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Plants were grown under six following different environmental regimes in greenhouse, Environment 1) 24oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 2) 24oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 3) 37oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 4) 37oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 5) 37oC/17oC day/night plus drought, with post-anthesis fertilizer, Environment 6) 37oC/17oC day/night plus drought, without post-anthesis fertilizer, developing wheat grains from the following were excised and frozen in liquid nitrogen, Environment 1 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 2 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 3 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 34 DPA Environment 4 at 3, 5, 7, 8, 10, 12, 16, 20, 24 , 28, 32, 34 DPA Environment 5 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 30 DPA Environment 6 at 3, 5, 7, 8, 10, 12, 16 , 20, 24, 28, 30 DPA and total RNA was prepared by S. Altenbach and K. Cronin at USDA-ARS, Albany, CA. A cDNA library was made using poly (A) RNA, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Chin, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (others). Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Plants were grown under six following different environmental regimes in greenhouse, Environment 1) 24oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 2) 24oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 3) 37oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 4) 37oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 5) 37oC/17oC day/night plus drought, with post-anthesis fertilizer, Environment 6) 37oC/17oC day/night plus drought, without post-anthesis fertilizer, developing wheat grains from the following were excised and frozen in liquid nitrogen, Environment 1 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 2 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 3 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 34 DPA Environment 4 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 34 DPA Environment 5 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 30 DPA Environment 6 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 30 DPA and total RNA was prepared by S. Altenbach and K. Cronin at USDA-ARS, Albany, CA. A cDNA library was made using poly (A) RNA, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Chin, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (others).
DNA
gaggggtgcgcaagccgccgcccaggaaccgggaccggcacaccaaggtg
gagggccgcggccgccggatccgcatgccggccgcctgcgccgccaggat
cttccagctcacccgcgagctagggcacaagtcggacggcgagaccgtgc
gctggctgctgcagcagtccgagccggccatcgtcgccgccacgggcacc
ggcaccgtgcccgccatcgccaccaccgtcgacggcgtgctccggatccc
cacccagtcctcctcctccgcctcctccgccgtcgtcgacgacgaggcct
ccgccaagcgccgccgcaagctgcagccccgnctgcgccgcggggccgct
cgccaccgtgccgcccgccgccgcctactactccgtcctcgccgaccccc
tcctgcagggcgngcgcccgggggcgcggccatttccgtcgccagcggcc
tcgctcccatcggcggccagcagggcctcgtccccgtcttcgccgtgccg
tccgccgctggggccggcggcggcaaccacatgatcccgcaggccacctg
ccgtgtggatggtcccgcagccggccggcgcccccaaccagcccacccag
tactgggccttccagtccgcgccccagctcatcaacctcgccggcgcgca
gaccatgcctggctggagctgtgttccccaacgtcgccgccgactaccat
caacagcagcagtctgcctctaccgtggt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ838703
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||