GrainGenes Sequence Report: NSFT03P2_Contig12464
Sequence
BQ838624
Contig
Ta.205.1.S1_at NSFT03P2_Contig12464
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC284418
NCBI UniGene
Ta.41909
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Hypothetical LOC542816'
Keyword
EST
Species
Triticum aestivum
Cultivar
BH1146
Clone Library
Wheat aluminum-stressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2912_G08_N16ZS Wheat aluminum-stressed root tip cDNA library Triticum aestivum cDNA clone WHE2912_G08_N16, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2912_G08_N16
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tactacagctactcgcagcccacccagccggctcccgcggcggaacccag
cccagcgccgcttccctatgatgccccgtactacggtggaggctaccagc
agcccgccgctgggtatggcgacgatgattacctgaacgagggcgcgtat
gcctatacaggcaacgcgggccccgagccgtacggcgcgcggagcacagc
gccgacgcgatcaggagccgcgatgtttgatgactacggccggtcgattg
gactctcgtccggaggggcggagcagccacacggcggcgggggcggcagc
gtgggtgggagctttgggaagattgccagggctgttccaaaagcagaaac
ccatgaggatgctagtggtggtgcgcagaagtttcgggttaagctgctgc
cagagggtgccggaagccctaccgatgtgctttgccaggttggtctggat
ggaatccgcatgcttgatcccagcacaaacaggaccctaaggatttatcc
ccttgatacactgaccaaatgggaggttttggattcaactgtatttgcga
tttgggcaaagacttcagtggattttga
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig12464
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||