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GrainGenes Sequence Report: BQ838450

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Sequence
BQ838450
Contig
Ta.14590.1.S1_a_at
Ta.14590.1.S1_at
Ta.14590.1.S1_x_at
NSFT03P2_Contig13361
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC282338
NCBI UniGene
Ta.54599
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001057176.1 Os06g0221200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
BH1146
Clone Library
Wheat aluminum-stressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2910_F02_K04ZS Wheat aluminum-stressed root tip cDNA library Triticum aestivum cDNA clone WHE2910_F02_K04, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2910_F02_K04
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ggcgcccgatggcgacgctcaaggtcccctccaacgtcccggccctcgcc
gacgactgcgacaacctccgcaaggccttccaagggtggggcacgaacga
ggcgctcatcatctccatcctcggccaccgcgacgccgcgcagcgccgcg
ccatccgcaagcactacgccgacacctacggcgaggagctgctccggagc
atcaccgacgagatctccggcgacttcgagagggccgtgatcctgtggac
gctggacccggcggagcgggacgcggtgctcgccaacgagaccgtcaaga
agtggcaccccgggaaccccgtgctggtcgagatcgcctgcgcgcgcggc
tccaagcagctcttcgctgtcaggcaggcctaccacgaccgcttcaagcg
ctcgctcgaggaggacgtcgctgcccatatcaccggcgacttccgcaagc
tgttggtgccacttgtaagctcacaccgctatgagggaccagaggtcaac
acaaggttggctcattcagaggccaaattactacatgagaagattgagca
taaggcttatggtgatgatgaggtcatcagaattctcaccactaggagca
aagctcaacattc

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