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GrainGenes Sequence Report: BQ838243

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Sequence
BQ838243
Contig
TaAffx.12194.1.S1_at
TaAffx.12194.3.S1_s_at
NSFT03P2_Contig8070
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC317351
NCBI UniGene
Ta.34360
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001055453.1 Os05g0393100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
BH1146
Clone Library
Wheat aluminum-stressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2908_B08_D16ZS Wheat aluminum-stressed root tip cDNA library Triticum aestivum cDNA clone WHE2908_B08_D16, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2908_B08_D16
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
attcggcaccagcagaatgcggcctctcctcagcgccgccgccgccgccg
ctgtcctcctcctcctcctccccctcgccgccggccagcgactccgcggc
ttctcctaccagctcgactgcggcgccgcgagctcctccaccgacagccg
cggcctccgctgggaccccgacggcccctacgtctccgccggctccgccc
gcccgctctccgtccagggcctcctcgaccccgccctcgcctccctccgc
gccttcccgtaccgcaccaacgccaagttctgctacgcgctccccgtcga
ccccaaccgccgctacctcctccgccccaccttcttctacggccccacct
cgacggccccgcccgtgttcgacctcatcgtggacggcaccttctggacc
gccgtcgacaccgccgatgacgtcctggccggcagcgcctcgcactacga
ggccgtgttcccggccaggggcaggaacctgaccttctgccttggcgtca
accccgactacacgaaatccggcccgttcatcaacgcgctgcaggtcatc
cagctccatgattcggtgtacaacgccacggatttcacgggcagcgccat
gggcctcattgcgcgcaccaagtttggctcgactggcgacgtcgagaggt
accctgatgacagttttgaccgctattggcagccatttccagacagaaaa
catgcagtcagtagtacccacaacgttacctcagccgatttctggaatct
tcctcctcctgacgtatttaatacagctttggtagcagaacaaatttcac
ccttg

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